| Title: |
Gene expression profiling in several types of stem cells; some cells treated with indomethacin in differentiation medium. [original title: DNA methylation in progenitor cells: expression study]
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| Organism(s): |
Homo sapiens
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| Description: |
Gene expression profiling of adipose stem cells (ASCs), bone marrow mesenchymal stem cells (BMMSCs) and muscle progenitor cells (MPCs). APCs were profiled either as primary cultures or after being induced to differentiate by adding indomethacin, insulin, dexamethasone, and IBMX to their growth medium. 11 samples, including 2 each of differentiated and undifferentiated ASCs. [original description: We surveyed DNA methylation profiles of all human RefSeq promoters in relation to gene expression and differentiation in adipose tissue, bone marrow and muscle mesenchymal progenitors, as well as in bone marrow-derived hematopoietic progenitors. We unravel strongly overlapping DNA methylation profiles between adipose stem cells (ASCs), bone marrow mesenchymal stem cells (BMMSCs) and muscle progenitor cells (MPCs), while hematopoietic progenitor cells (HPCs) are more epigenetically distant from MSCs seen as a whole. Differentiation resolves a fraction of methylation patterns common to MSCs, generating epigenetic divergence. RNA was isolated from MSCs isolated from various tissues and from differentiated cells, and hybridized onto Illumina expression arrays. 2-3 replicates per cell type. The supplementary file 'GSE19773_non-normalized.txt' contains the non-normalized data for Samples GSM493884-GSM493894.]
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| Design(s): |
transcription profiling by array
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| Experimental factor(s): |
|
| indomethacin
2
recorded
|
|
|
|
none
indometacin
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| differentiation
2
recorded
|
|
|
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differentiated
primary culture
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| organism part
4
recorded
|
|
|
|
not specified
adipose tissue
bone marrow
skeletal muscle
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| primary culture cell type
3
recorded
|
|
|
|
muscle stem cell
adipose-derived stem cell
mesenchymal stem cell of the bone marrow
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| cell type
3
recorded
|
|
|
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myogenic-differentiated mesenchymal stem cells
adipogenic-differentiated mesenchymal stem cells
mesenchymal stem cells
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| source tissue
3
recorded
|
|
|
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skeletal muscle
adipose tissue
bone marrow
|
|
| Publication(s): |
Srensen AL, Jacobsen BM, Reiner AH, Andersen IS, Collas P
Promoter DNA methylation patterns of differentiated cells are largely programmed at the progenitor stage.
PubMed:20410135
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| Sample attribute(s): |
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| Material Type
1
recorded
|
|
|
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total RNA
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| differentiation
3
recorded
|
|
|
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in vitro differentiated myocytes
in vitro differentiated adipocytes
primary culture
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| organism part
3
recorded
|
|
|
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adipose tissue
bone marrow
skeletal muscle
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| Label
1
recorded
|
|
|
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biotin
|
| cell type
3
recorded
|
|
|
|
myogenic-differentiated mesenchymal stem cells
adipogenic-differentiated mesenchymal stem cells
mesenchymal stem cells
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| Organism
1
recorded
|
|
|
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Homo sapiens
|
|
| Contact(s): |
Bente Jacobsen Andrew ReinerPhilippe Collas
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| Release Date: |
5/11/2010
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| Submission Date: |
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| Metadata Downloads |
Download Study Metadata as ISAtabDownload Study Metadata as ISAtab
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| Assay Downloads |
| transcription profiling using DNA microarray
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| 11 assays |
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