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E-GEOD-19773

Title: Gene expression profiling in several types of stem cells; some cells treated with indomethacin in differentiation medium. [original title: DNA methylation in progenitor cells: expression study]
Organism(s): Homo sapiens
Description: Gene expression profiling of adipose stem cells (ASCs), bone marrow mesenchymal stem cells (BMMSCs) and muscle progenitor cells (MPCs). APCs were profiled either as primary cultures or after being induced to differentiate by adding indomethacin, insulin, dexamethasone, and IBMX to their growth medium. 11 samples, including 2 each of differentiated and undifferentiated ASCs. [original description: We surveyed DNA methylation profiles of all human RefSeq promoters in relation to gene expression and differentiation in adipose tissue, bone marrow and muscle mesenchymal progenitors, as well as in bone marrow-derived hematopoietic progenitors. We unravel strongly overlapping DNA methylation profiles between adipose stem cells (ASCs), bone marrow mesenchymal stem cells (BMMSCs) and muscle progenitor cells (MPCs), while hematopoietic progenitor cells (HPCs) are more epigenetically distant from MSCs seen as a whole. Differentiation resolves a fraction of methylation patterns common to MSCs, generating epigenetic divergence. RNA was isolated from MSCs isolated from various tissues and from differentiated cells, and hybridized onto Illumina expression arrays. 2-3 replicates per cell type. The supplementary file 'GSE19773_non-normalized.txt' contains the non-normalized data for Samples GSM493884-GSM493894.]
Design(s): transcription profiling by array
Experimental factor(s):
indomethacin
2 recorded
none indometacin
differentiation
2 recorded
differentiated primary culture
organism part
4 recorded
not specified adipose tissue bone marrow skeletal muscle
primary culture cell type
3 recorded
muscle stem cell adipose-derived stem cell mesenchymal stem cell of the bone marrow
cell type
3 recorded
myogenic-differentiated mesenchymal stem cells adipogenic-differentiated mesenchymal stem cells mesenchymal stem cells
source tissue
3 recorded
skeletal muscle adipose tissue bone marrow
Publication(s): S¬Ěrensen AL, Jacobsen BM, Reiner AH, Andersen IS, Collas P
Promoter DNA methylation patterns of differentiated cells are largely programmed at the progenitor stage. PubMed:20410135

Sample attribute(s):
Material Type
1 recorded
total RNA
differentiation
3 recorded
in vitro differentiated myocytes in vitro differentiated adipocytes primary culture
organism part
3 recorded
adipose tissue bone marrow skeletal muscle
Label
1 recorded
biotin
cell type
3 recorded
myogenic-differentiated mesenchymal stem cells adipogenic-differentiated mesenchymal stem cells mesenchymal stem cells
Organism
1 recorded
Homo sapiens
Contact(s): Bente JacobsenAndrew ReinerPhilippe Collas
Release Date: 5/11/2010
Submission Date:
Metadata Downloads Download Study Metadata as ISAtabDownload Study Metadata as ISAtab
Assay Downloads
transcription profiling using DNA microarray
11 assays