Title: |
Transcriptional profiling of mouse synovial fibroblasts treated with indomethacin or iloprost [original title: Transcription profiling of synovial mouse fibroblast activated by IL-1beta effect of prostacyclin signaling]
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Organism(s): |
Mus musculus
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Description: |
Synovial fibroblasts were extracted from knee joints of naíve mice (DBA/1J, male 8-12 week old), and passaged 3-4 times. Cells were plated at a concentration of 1 X 10^6 cells/dish in 60 mm dish in 5 ml of RPMI1640 medium with 1% heat-inactivated FCS at 37°C, 5% CO2. Cells were treated with vehicle or stimulated by IL-1beta. In IL-1beta (5ng/ml) stimulated groups, indomethacin (1 µM) and iloprost (1 µM) were further added either alone or in combination. After 6 hours of stimulation as described above, cells were isolated and frozen by liquid nitrogen, and stored at -80°C before use. Total RNA was extracted by RNAeasy Mini Kit. The RNA sample was labeled for hybridization onto the genechip array according to the standard affymetrix protocols.
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Design(s): |
compound_treatment_design, transcription profiling by array
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Experimental factor(s): |
compound
4
recorded
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none
IL-1beta, indomethacin, iloprost
IL-1beta, indomethacin
IL-1beta
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Publication(s): |
Tetsuya Honda, Eri Segi-Nishida, Yoshiki Miyachi, Shuh Narumiya
Prostacyclin-IP signaling and prostaglandin E2-EP2/EP4 signaling both mediate joint inflammation in mouse collagen-induced arthritis.
PubMed:16446378
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Sample attribute(s): |
IL-1 beta conc
2
recorded
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0 ng/ml
5 ng/ml
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Material Type
2
recorded
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synthetic_RNA
total_RNA
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sex
1
recorded
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male
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IL-1 beta treatment
2
recorded
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placebo
interleukin-1 beta
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iloprost conc
2
recorded
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0 micromolar
1 micromolar
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strain
1
recorded
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DBA/1J Mouse
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iloprost treatment
2
recorded
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placebo
iloprost
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indomethacin treatment
2
recorded
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placebo
indometacin
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organism
1
recorded
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Mus musculus
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culture type
1
recorded
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primary culture
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indomethacin conc
2
recorded
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0 micromolar
1 micromolar
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age
1
recorded
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8-12 week
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Label
1
recorded
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biotin
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cell type
1
recorded
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synovial fibroblast
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source tissue
1
recorded
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knee
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Contact(s): |
tetsuya honda
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Release Date: |
6/12/2008
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Submission Date: |
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Metadata Downloads |
Download Study Metadata as ISAtabDownload Study Metadata as ISAtab
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Assay Downloads |
transcription profiling using DNA microarray
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4 assays |
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