Title: Transcriptional profiling of PC3 prostate cancer cells treated with the NSAIDs sulindac or nitrosulindac (aka NO-sulindac, NCX-1102). [original title: Effect of NO-sulindac treatment on hypoxic prostate cancer cells]
Organism(s): Homo sapiens
Description: Cells were grown either in hypoxic or normoxic conditions and treated either with NSAID (sulindac or nitrosulindac) or control media (DMSO only or no drug). [original description: The hypoxia response contributes to radio and chemo-resistance in cancer cells. Our previous work has shown that the nitric oxide donating non-steroidal anti-inflammatory drug (NO-NSAID) NO-sulindac is a potent inhibitor of the hypoxia response in prostate cancer cells and leads to increased susceptibility to radiation. In this study we used microarrays to investigated the global impact of NO-sulindac on the hypoxia response in prostate cancer cells with a view to determining the mechanism of action. PC3 hormone-insensitive prostate cancer cells were grown under normoxic or hypoxic conditions and treated with NO-sulindac, unnitrated sulindac or vehicle control. Global gene expression in response to treatment was examined using microarrays and the bioconductor software suite. Gene set enrichment analysis (GSEA), Gene ontology (GO) analysis and pathway analysis were used to examine the biological impact of treatments.]
Design(s): transcription profiling by array
Experimental factor(s):
NSAID concentration
1 recorded
25 micromolar
4 recorded
control condition nitrosulindac dimethyl sulfoxide sulindac
environmental condition
2 recorded
hypoxia normoxia
oxygen level
2 recorded
0.2% 21%
oxygen level description
2 recorded
decreased concentration normal
Sample attribute(s):
cell line
1 recorded
PC-3 cell
1 recorded
1 recorded
Homo sapiens
oxygen level
2 recorded
0.2% 21%
Contact(s): Iain GallagherIain Gallagher
Release Date: 12/1/2012
Submission Date:
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Assay Downloads
transcription profiling using DNA microarray
16 assays