| Title: |
Transcriptional profiling of PC3 prostate cancer cells treated with the NSAIDs sulindac or nitrosulindac (aka NO-sulindac, NCX-1102). [original title: Effect of NO-sulindac treatment on hypoxic prostate cancer cells]
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| Organism(s): |
Homo sapiens
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| Description: |
Cells were grown either in hypoxic or normoxic conditions and treated either with NSAID (sulindac or nitrosulindac) or control media (DMSO only or no drug). [original description: The hypoxia response contributes to radio and chemo-resistance in cancer cells. Our previous work has shown that the nitric oxide donating non-steroidal anti-inflammatory drug (NO-NSAID) NO-sulindac is a potent inhibitor of the hypoxia response in prostate cancer cells and leads to increased susceptibility to radiation. In this study we used microarrays to investigated the global impact of NO-sulindac on the hypoxia response in prostate cancer cells with a view to determining the mechanism of action. PC3 hormone-insensitive prostate cancer cells were grown under normoxic or hypoxic conditions and treated with NO-sulindac, unnitrated sulindac or vehicle control. Global gene expression in response to treatment was examined using microarrays and the bioconductor software suite. Gene set enrichment analysis (GSEA), Gene ontology (GO) analysis and pathway analysis were used to examine the biological impact of treatments.]
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| Design(s): |
transcription profiling by array
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| Experimental factor(s): |
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| NSAID concentration
1
recorded
|
|
|
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25 micromolar
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| treatment
4
recorded
|
|
|
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control condition
nitrosulindac
dimethyl sulfoxide
sulindac
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| environmental condition
2
recorded
|
|
|
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hypoxia
normoxia
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| oxygen level
2
recorded
|
|
|
|
0.2%
21%
|
| oxygen level description
2
recorded
|
|
|
|
decreased concentration
normal
|
|
| Publication(s): |
|
| Sample attribute(s): |
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| cell line
1
recorded
|
|
|
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PC-3 cell
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| Label
1
recorded
|
|
|
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biotin
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| organism
1
recorded
|
|
|
|
Homo sapiens
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| oxygen level
2
recorded
|
|
|
|
0.2%
21%
|
|
| Contact(s): |
Iain Gallagher Iain Gallagher
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| Release Date: |
12/1/2012
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| Submission Date: |
|
| Metadata Downloads |
Download Study Metadata as ISAtabDownload Study Metadata as ISAtab
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| Assay Downloads |
| transcription profiling using DNA microarray
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| 16 assays |
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