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E-GEOD-36010

Title: Expression profiling of rat cerebral cortex after surgical induction of ischemia/infarction; the NSAID meloxicam was used during surgical prep. [original title: mRNA profiles after experimental ischemia]
Organism(s): Rattus norvegicus
Description: To analyze gene expression we isolated total mRNA from the core, periinfarct and contralateral cortex of 60 Sprague-Dawley rats after 24 hours or 3 days of permanent middle cerebral artery occlusion (pMCAo). Also ipsilateral and contralateral cortex was obtained from sham-operated and healthy animals. A total of 44 microarrays were performed with RNA pooled from 3 independent animals. For each experimental condition the total number is 12 [rats]. Microarray analysis of different areas of the rat brain (core, periinfarct and contralateral cortex) after 24h and 3 days of permanent ischemia.
Design(s): transcription profiling by array
Experimental factor(s):
time
3 recorded
3d 24h 0h
treatment
3 recorded
infarct sham healthy
brain region
4 recorded
Contralateral area Core area Periinfarct area Ipsilateral area
rat
20 recorded
12 10 3 9 1 8 13 5 4 14 7 11 2 15 17 20 16 19 6 18
Publication(s): Ramos-Cejudo J, Gutiérrez-Fernández M, Rodríguez-Frutos B, Expósito Alcaide M, Sánchez-Cabo F, Dopazo A, Díez-Tejedor E.
Spatial and temporal gene expression differences in core and periinfarct areas in experimental stroke: a microarray analysis. PubMed:23284893

Sample attribute(s):
ketamine
1 recorded
NSAID
1 recorded
meloxicam ChEBI 6741
strain or line
1 recorded
Sprague Dawley
brain region
4 recorded
Contralateral area Core area Periinfarct area Ipsilateral area
atropine
1 recorded
rat
20 recorded
12 10 3 9 1 8 13 5 4 14 7 11 2 15 17 20 16 19 6 18
organism
1 recorded
Rattus norvegicus
proximity to infarction
2 recorded
located in adjacent to
diazepam
1 recorded
side
2 recorded
left of right of
organism part
1 recorded
cerebral cortex
ischemia
1 recorded
brain
Label
1 recorded
Cy3
Contact(s): María Gutiérrez-FernándezJaime CejudoExuperio Díez-TejedorAna DopazoJaime Ramos-CejudoBerta Rodríguez-FrutosMercedes AlcaideFátima Sánchez-Cabo
Release Date: 12/1/2012
Submission Date:
Metadata Downloads Download Study Metadata as ISAtabDownload Study Metadata as ISAtab
Assay Downloads
transcription profiling using DNA microarray
44 assays