| Title: |
Expression profiling of rat cerebral cortex after surgical induction of ischemia/infarction; the NSAID meloxicam was used during surgical prep. [original title: mRNA profiles after experimental ischemia]
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| Organism(s): |
Rattus norvegicus
|
| Description: |
To analyze gene expression we isolated total mRNA from the core, periinfarct and contralateral cortex of 60 Sprague-Dawley rats after 24 hours or 3 days of permanent middle cerebral artery occlusion (pMCAo). Also ipsilateral and contralateral cortex was obtained from sham-operated and healthy animals. A total of 44 microarrays were performed with RNA pooled from 3 independent animals. For each experimental condition the total number is 12 [rats]. Microarray analysis of different areas of the rat brain (core, periinfarct and contralateral cortex) after 24h and 3 days of permanent ischemia.
|
| Design(s): |
transcription profiling by array
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| Experimental factor(s): |
|
| time
3
recorded
|
|
|
|
3d
24h
0h
|
| treatment
3
recorded
|
|
|
|
infarct
sham
healthy
|
| brain region
4
recorded
|
|
|
|
Contralateral area
Core area
Periinfarct area
Ipsilateral area
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| rat
20
recorded
|
|
|
|
12
10
3
9
1
8
13
5
4
14
7
11
2
15
17
20
16
19
6
18
|
|
| Publication(s): |
Ramos-Cejudo J, Gutiérrez-Fernández M, Rodríguez-Frutos B, Expósito Alcaide M, Sánchez-Cabo F, Dopazo A, Díez-Tejedor E.
Spatial and temporal gene expression differences in core and periinfarct areas in experimental stroke: a microarray analysis.
PubMed:23284893
|
| Sample attribute(s): |
|
| ketamine
1
recorded
|
|
|
|
|
| NSAID
1
recorded
|
|
|
|
meloxicam ChEBI 6741
|
| strain or line
1
recorded
|
|
|
|
Sprague Dawley
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| brain region
4
recorded
|
|
|
|
Contralateral area
Core area
Periinfarct area
Ipsilateral area
|
| atropine
1
recorded
|
|
|
|
|
| rat
20
recorded
|
|
|
|
12
10
3
9
1
8
13
5
4
14
7
11
2
15
17
20
16
19
6
18
|
| organism
1
recorded
|
|
|
|
Rattus norvegicus
|
| proximity to infarction
2
recorded
|
|
|
|
located in
adjacent to
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| diazepam
1
recorded
|
|
|
|
|
| side
2
recorded
|
|
|
|
left of
right of
|
| organism part
1
recorded
|
|
|
|
cerebral cortex
|
| ischemia
1
recorded
|
|
|
|
brain
|
| Label
1
recorded
|
|
|
|
Cy3
|
|
| Contact(s): |
María Gutiérrez-Fernández Jaime CejudoExuperio Díez-TejedorAna DopazoJaime Ramos-CejudoBerta Rodríguez-FrutosMercedes AlcaideFátima Sánchez-Cabo
|
| Release Date: |
12/1/2012
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| Submission Date: |
|
| Metadata Downloads |
Download Study Metadata as ISAtabDownload Study Metadata as ISAtab
|
| Assay Downloads |
| transcription profiling using DNA microarray
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|
| 44 assays |
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