Title: Time course of gene expression during adipogenesis. Adipogenic induction medium contained indomethacin. [original title: Differentially expressed zinc finger proteins during human adipogenesis]
Organism(s): Homo sapiens
Description: To investigate the collective role of zinc finger proteins in adipogenesis, we induced adipogenesis using human mesenchymal stem cells (MSC) and compared the gene expression profiles using human Illumina beads arrays. We performed three independent experiments of time-series analysis (0h, 1h, 12h, 24h, 2d, 4d, 6d, 9d, 12d, 15d after induction of adipogenesis) in order to comprehensively capture the dynamic profiles of transcriptome during differentiation. Statistical filtering based on Analysis of Variance (ANOVA; p-value < 0.001, FDR 5%) and Gene Ontology classification using the Human Gene Nomenclature Database (HGNC) identified 618 out of 678 zinc finger proteins (ZFPs) that were significantly expressed during adipogenesis. Moreover, 34 out of 618 genes were differentially expressed more than 2-fold up or down regulated. The K-means clustering analysis further revealed four dynamic expression patterns: one gene in early-response, seven genes in transient, twenty genes in progressively-induced and four genes in the down-regulated, demonstrating the dynamic involvement of ZFPs in the process of adipocyte differentiation and maturation. Total RNA obtained from 10 different time points (0h, 1h, 12h, 24h, 2d, 4d, 6d, 9d, 12d, 15d) during adipogenesis from human Mesenchymal stem cell (MSC) to Adipocyte (ADP). Adipogenesis was induced by the different cocktail of chemical compounds containing human-insulin, dexamethasone, indomethacin and IBMX.
Design(s): transcription profiling by array
Experimental factor(s):
time after induction
10 recorded
9day 2day 0hr 1day 15day 1hr 12day 12hr 6day 4day
time after induction - hours
10 recorded
216 hour 48 hour 0 hour 24 hour 360 hour 1 hour 288 hour 12 hour 144 hour 96 hour
Sample attribute(s):
drug 1
1 recorded
time after induction
10 recorded
9day 2day 0hr 1day 15day 1hr 12day 12hr 6day 4day
drug 2
1 recorded
insulin (human)
drug 3
1 recorded
drug 1 type
1 recorded
non-steroidal anti-inflammatory drug
drug 4
1 recorded
1 recorded
cell type
1 recorded
mesenchymal stem cell
1 recorded
Homo sapiens
Contact(s): H YoshihideA OgawaH SuzukiRyota HasegawaJay ShinH Ryota
Release Date: 12/1/2012
Submission Date:
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Assay Downloads
transcription profiling using DNA microarray
30 assays