| Title: |
Transcription profiling time series of the response of Rattus norvegicus colon carcinoma cell line CC531 to treatment with butyrate or aspirin
|
| Organism(s): |
Rattus norvegicus
|
| Description: |
Temporal analysis of colon carcinoma cell line CC531 response to 4.5 mM butyrate or 3 mM aspirin. Samples taken at 2, 6, 12, 16 and 24 hours. The CC531 cell line has been widely used to study different aspects of tumor growth and metastasis and provides an excellent experimental platform to develop novel antitumor strategies. To characterize the CC531 model at the molecular level, we screened for mutations in genes covering important signal-transduction pathways that are known to play major roles during colon carcinogenesis, the wnt and the ki-ras signaling pathways. We found both a prototypic beta-catenin (Ctnnb1) mutation (Thr(41)Ile) and a ki-ras (G12D) mutation, providing unambiguous evidence for the constitutive activation of these pathways in CC531 cells. We further established comprehensive gene expression profiles of CC531 cells and investigated the molecular response to 2 antitumor drugs, butyrate and aspirin. Using oligonucleotide microarrays, we screened the expression levels of 7,700 genes and identified a total of 398 genes whose expression was significantly changed upon treatment with butyrate. When using aspirin, 121 genes were significantly altered. Interestingly, 36 genes were regulated by both butyrate and aspirin and 35 of them were regulated in the same direction. We found 7 differentially expressed genes, cyclin D1, cyclin E, c-myc, Fosl1, c-fos, Cd44 and follistatin, which are known targets of the beta-catenin and/or the ras pathway. In all cases, butyrate and aspirin reversed the changes in expression normally found in response to active signaling of these oncogenic pathways.
|
| Design(s): |
transcription profiling by array
|
| Experimental factor(s): |
|
| time
10
recorded
|
|
|
|
6
6 hour
24 hour
24
2
2 hour
12 hour
12
16
16 hour
|
| compound
3
recorded
|
|
|
|
acetylsalicylic acid
butyrate
control condition
|
| dose
4
recorded
|
|
|
|
3
3 millimolar
4.5 millimolar
4.5
|
|
| Publication(s): |
Anja Germann, Susanne Dihlmann, Manfred Hergenhahn, Magnus von Knebel Doeberitz, Robert Koesters
Expression profiling of CC531 colon carcinoma cells reveals similar regulation of beta-catenin target genes by both butyrate and aspirin.
PubMed:12800193
|
| Sample attribute(s): |
|
| CellLine
1
recorded
|
|
|
|
CC531
|
| Material Type
2
recorded
|
|
|
|
synthetic_RNA
total_RNA
|
| DiseaseState
1
recorded
|
|
|
|
colonic cancer cell line
|
| Label
1
recorded
|
|
|
|
biotin
|
| organism
1
recorded
|
|
|
|
Rattus norvegicus
|
|
| Contact(s): |
Anja Germann
|
| Release Date: |
10/10/2007
|
| Submission Date: |
|
| Metadata Downloads |
Download Study Metadata as ISAtabDownload Study Metadata as ISAtab
|
| Assay Downloads |
| transcription profiling using DNA microarray
|
|
| 12 assays |
|
|
|
|
