Title: Chip-chip from undifferentiated and neuron-differentiated hMSCs cells with EZH2
Organism(s): Homo sapiens
Description: Half the samples were treated with indomethacin and several other chemicals. [original description: EZH2 plays an important role in stem cell renewal and maintenance by inducing gene silencing via its histone methyltransferase activity. Previously, we showed EZH2 downregulation markedly enhances neuron differentiation of human mesenchymal stem cells (hMSCs). To understand how EZH2 regulates neuron differentiation of hMSCs, we wanted to identify the target genes of EZH2. For this reasons we performed ChIP-on-chip experiments using specific EZH2 antibodies followed by a human promoter array for the whole human genome. The 3A6-hMSCs were differentiated into neuron for 5 days, and then 109 cells were harvested for the ChIP-on-chip assay. The procedure was based on the manufacturer's instructions (NimbleGen).]
Design(s): ChIP-chip design
Publication(s): Yu YL, Chou RH, Shyu WC, Hsieh SC, Wu CS, Chiang SY, Chang WJ, Chen JN, Tseng YJ, Lin YH, Lee W, Yeh SP, Hsu JL, Yang CC, Hung SC, Hung MC.
Smurf2-mediated degradation of EZH2 enhances neuron differentiation and improves functional recovery after ischaemic stroke. PubMed:23526793

Sample attribute(s):
drug 1
1 recorded
NSAID treatment
1 recorded
drug 2
1 recorded
ascorbic acid-2-phosphate
cell line type
1 recorded
mesenchymal stem cell
cell line
1 recorded
chemical 1
1 recorded
2 recorded
Cy3 Cy5
chemical 2
1 recorded
cell type
2 recorded
neuron-differentiated hMSCs undifferentiated hMSCs
1 recorded
Homo sapiens
Contact(s): chanshiou WuYung-Luen YuChen-Shiou Wu
Release Date: 12/5/2012
Submission Date:
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Assay Downloads
protein-DNA binding site identification using DNA microarray
4 assays