| Title: |
Transcription profiling of PBMCs from patients with several autoimmune inflammatory diseases or similar disorders and healthy controls. Some patients are on Celebrex or steroids. [original title: Transcription profiling of human blood leukocytes to diagnose systemic onset juvenile idiopathic arthritis and follow IL-1 blockade]
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| Organism(s): |
Homo sapiens
|
| Description: |
123 patients, duplicate samples. [original description: Systemic onset Juvenile Idiopathic Arthritis (SoJIA) represents up to 20% of Juvenile Idiopathic Arthritis (JIA). We have previously reported that this disease is Interleukin 1 (IL1)-mediated, and that IL-1 blockade results in clinical remission in the majority of patients. The diagnosis of SoJIA, however, still relies on clinical findings as no specific diagnostic tests are available, which leads to delays in the initiation of specific therapy. To identify specific diagnostic markers, we analyzed gene expression profiles in 19 pediatric patients with SoJIA during the systemic phase of the disease (fever and/or arthritis), 25 SoJIA patients with no systemic symptoms (arthritis only or no symptoms), 39 healthy controls, 94 pediatric patients with acute viral and bacterial infections (available under GSE6269), 38 pediatric patients with Systemic Lupus Erythematosus (SLE), and 6 patients with a second IL-1 mediated disease known as PAPA syndrome. Statistical group comparison and class prediction identified genes differentially expressed in SoJIA patients compared to healthy children. These genes, however, were also changed in patients with acute infections and SLE. By performing an analysis of significance across all diagnostic groups, we generated a list of 88 SoJIA-specific genes (p<0.01 in SoJIA and >0.5 in all other groups). A subset of 12/88 genes permitted us to accurately classify an independent test set of SoJIA patients with systemic disease. We were also able to identify a group of transcripts that changed significantly in patients undergoing IL-1 blockade. Thus, analysis of transcriptional signatures from SoJIA blood leukocytes can help distinguishing this disease from other febrile illnesses and assessing response to therapy. Availability of accurate diagnostic markers for SoJIA patients may allow prompt initiation of effective therapy and prevention of long-term disabilities. Experiment Overall Design: 123 RNA samples extracted from PBMCs were studied. For more details on the clinical information, please refer to the paper (PudMed ID 17724127).]
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| Design(s): |
unknown_experiment_design_type, transcription profiling by array
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| Publication(s): |
Allantaz F, Chaussabel D, Stichweh D, Bennett L, Allman W, Mejias A, Ardura M, Chung W, Smith E, Wise C, Palucka K, Ramilo O, Punaro M, Banchereau J, Pascual V.
Blood leukocyte microarrays to diagnose systemic onset juvenile idiopathic arthritis and follow the response to IL-1 blockade.
PubMed:17724127
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| Sample attribute(s): |
|
| drug2
6
recorded
|
|
|
|
azathioprine
interleukin-1 receptor antagonist protein
ChEBI infliximab
cyclophosphamide
ENBREL
mycophenolic acid
|
| drug3
1
recorded
|
|
|
|
amlodipine
|
| Material Type
2
recorded
|
|
|
|
synthetic_RNA
total_RNA
|
| NSAID treatment
1
recorded
|
|
|
|
celecoxib
|
| sex
2
recorded
|
|
|
|
female
male
|
| drug1
1
recorded
|
|
|
|
methotrexate
|
| race
5
recorded
|
|
|
|
Caucasian
Latino
Other
Asian
African American
|
| SLEDAI score
12
recorded
|
|
|
|
2 dimensionless
8 dimensionless
20 dimensionless
6 dimensionless
12 dimensionless
18 dimensionless
24 dimensionless
10 dimensionless
16 dimensionless
14 dimensionless
4 dimensionless
11 dimensionless
|
| illness
4
recorded
|
|
|
|
systemic lupus erythematosus
RHEUMATOID ARTHRITIS, SYSTEMIC JUVENILE
Healthy_control_subjects
PYOGENIC STERILE ARTHRITIS, PYODERMA GANGRENOSUM, AND ACNE
|
| symptom2
1
recorded
|
|
|
|
Fever
|
| age
20
recorded
|
|
|
|
16 years
10 years
15 years
8 years
13 years
6 years
4 years
17 years
2 years
11 years
9 years
19 years
14 years
5 years
7 years
20 years
68 years
12 years
40 years
3 years
|
| Steroid treatment2
1
recorded
|
|
|
|
Intravenous steroid injection
|
| Label
1
recorded
|
|
|
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biotin
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| steroid treatment
1
recorded
|
|
|
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Oral steroid therapy
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| cell type
1
recorded
|
|
|
|
peripheral blood mononuclear cell
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| Organism
1
recorded
|
|
|
|
Homo sapiens
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| steroid therapy
1
recorded
|
|
|
|
steroid
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| symptom
4
recorded
|
|
|
|
Systemic lupus erythematosus disease activity index
arthritis
None
Autoinflammatory syndrome with skin involvement
|
|
| Contact(s): |
Florence Allantaz
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| Release Date: |
6/16/2008
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| Submission Date: |
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| Metadata Downloads |
Download Study Metadata as ISAtabDownload Study Metadata as ISAtab
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| Assay Downloads |
| transcription profiling using DNA microarray
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| 246 assays |
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