| Title: |
Trancriptional profiling of five classes of white blood cells in patients with coronary artery disease. [original title: Circulating Mononuclear Cell Transcriptomes in Patients with Atherosclerotic Coronary Artery Disease]
|
| Organism(s): |
Homo sapiens
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| Description: |
18 patients with severe triple-vessel coronary artery disease (CAD) were matched to 13 control patients who had no sign of CAD but who had been admitted with chest pain and likelihood of CAD. All patients were on aspirin and statin treatment. The following types of cells were isolated: macrophages, resting monocytes, stem cells, stimulated monocytes, and T cells. Refer to supplementary methods for the detailed separation protocol and supplementary table 1 for additional details on patient characteristics including medical history and lab scores; due to ambiguities, however, it is not possible to distinguish patient A11 from A35 or patient A7 from A37. Patients were excluded if they had a myocardial infarction within the last four weeks, acute coronary syndrome, diabetes mellitus, neoplastic disease, or systemic inflammatory disease. [original description: Monocytes and T-cells play an important role in the development of atherosclerotic coronary artery disease (CAD). Differences in transcriptional activity of these cells might reflect the individual's atherosclerotic burden. Transcriptome analysis of circulating mononuclear cells from carefully matched atherosclerotic and control patients will potentially provide insights into the pathophysiology of atherosclerosis and supply biomarkers for diagnostic purposes. From patients undergoing coronary angiography because of anginal symptoms, we carefully matched 18 patients with severe triple-vessel CAD to 13 control patients without signs of CAD on angiography. All patients were on statin and aspirin treatment. RNA from circulating CD4+ T-cells, CD14+ monocytes, lipopolysaccharide-stimulated monocytes, macrophages and CD34+ progenitor cells was subjected to genome-wide expression analysis. Only CD14+ monocytes demonstrated that a small number of genes involved in activation was overexpressed in control patients, which was verified by real-time polymerase-chain reaction. In this pilot study, cautious matching of patients with severe atherosclerotic CAD with control patients without angiographic signs of coronary atherosclerosis did not reveal differences in transcriptional activity in four out of five different mononuclear cell types. In resting monocytes from patients without overt CAD some inflammatory genes were overexpressed as compared to patients with severe CAD. Large inter-individual variability prevented the use of single differentially expressed genes as biomarkers. Keywords: disease-state analysis In total 153 arrays were analyzed with 6 technical replicates (147 biological samples). CD34+ stem cells, CD4+ T-cells, resting CD14+ monocytes, stimulated monocytes and macrophages were analyzed, all from patient with severe coronary atherosclerosis or controls that had no coronary atherosclerosis as determined angiographically, and which were carefully matched for age and gender.]
|
| Design(s): |
transcription profiling by array
|
| Publication(s): |
Schirmer SH, Fledderus JO, van der Laan AM, van der Pouw-Kraan TC, Moerland PD, Volger OL, Baggen JM, Böhm M, Piek JJ, Horrevoets AJ, van Royen N
Suppression of inflammatory signaling in monocytes from patients with coronary artery disease.
PubMed:19059264
|
| Sample attribute(s): |
|
| Coronary artery disease
2
recorded
|
|
|
|
no
yes
|
| sex
2
recorded
|
|
|
|
female
male
|
| LPS incubation
2
recorded
|
|
|
|
20 hour
3 hour
|
| tissue
1
recorded
|
|
|
|
peripheral blood
|
| race
1
recorded
|
|
|
|
Caucasian
|
| Calcium Antagonists
2
recorded
|
|
|
|
no
calcium channel blocker
|
| family history of CAD
2
recorded
|
|
|
|
Family History of Coronary Artery Disease
no
|
| biopsy site
1
recorded
|
|
|
|
arterial sheath
|
| patient ID_REF
31
recorded
|
|
|
|
A8
A27
A6
A12
A7
A20
A11
A36
A35
A41
A10
A32
A25
A31
A2
A39
A15
A30
A9
A34
A38
A4
A1
A3
A13
A33
A19
A37
A5
A22
A24
|
| age
17
recorded
|
|
|
|
54 year
56 year
43 year
58 year
66 year
55 year
44 year
62 year
52 year
60 year
49 year
65 year
47 year
42 year
59 year
45 year
37 year
|
| ACE-Inhibitors/ Angiotensin receptor blockers
2
recorded
|
|
|
|
no
yes
|
| admitting diagnosis
2
recorded
|
|
|
|
Chest pain, unspecified
coronary artery disease
|
| aspirin
1
recorded
|
|
|
|
acetylsalicylic acid
|
| nitrates
2
recorded
|
|
|
|
no
Nitrate-based vasodilating agent
|
| diuretic
3
recorded
|
|
|
|
no
unknown
|
| organism
1
recorded
|
|
|
|
Homo sapiens
|
| replicate
1
recorded
|
|
|
|
rep2
|
| beta blocker
2
recorded
|
|
|
|
beta-adrenergic antagonist
no
|
| culture type
2
recorded
|
|
|
|
primary culture
primary cell
|
| statin
1
recorded
|
|
|
|
hydroxymethylglutaryl-CoA reductase inhibitor
|
| previous MI
4
recorded
|
|
|
|
no
History of - myocardial infarction
unknown
No
|
| smoker
3
recorded
|
|
|
|
Non-
Ex-
Smoker
|
| Label
1
recorded
|
|
|
|
biotin
|
| cell type
3
recorded
|
|
|
|
CD34
CD4
CD14
|
| cell type2
4
recorded
|
|
|
|
macrophage
common lymphoid progenitor
T-lymphocyte
monocyte
|
|
| Contact(s): |
Stephan Schirmer N RoyenStephan SchirmerAnton HorrevoetsJoost Fledderus
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| Release Date: |
12/1/2008
|
| Submission Date: |
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| Metadata Downloads |
Download Study Metadata as ISAtabDownload Study Metadata as ISAtab
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| Assay Downloads |
| transcription profiling using DNA microarray
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| 153 assays |
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