Title: Trancriptional profiling of five classes of white blood cells in patients with coronary artery disease. [original title: Circulating Mononuclear Cell Transcriptomes in Patients with Atherosclerotic Coronary Artery Disease]
Organism(s): Homo sapiens
Description: 18 patients with severe triple-vessel coronary artery disease (CAD) were matched to 13 control patients who had no sign of CAD but who had been admitted with chest pain and likelihood of CAD. All patients were on aspirin and statin treatment. The following types of cells were isolated: macrophages, resting monocytes, stem cells, stimulated monocytes, and T cells. Refer to supplementary methods for the detailed separation protocol and supplementary table 1 for additional details on patient characteristics including medical history and lab scores; due to ambiguities, however, it is not possible to distinguish patient A11 from A35 or patient A7 from A37. Patients were excluded if they had a myocardial infarction within the last four weeks, acute coronary syndrome, diabetes mellitus, neoplastic disease, or systemic inflammatory disease. [original description: Monocytes and T-cells play an important role in the development of atherosclerotic coronary artery disease (CAD). Differences in transcriptional activity of these cells might reflect the individual's atherosclerotic burden. Transcriptome analysis of circulating mononuclear cells from carefully matched atherosclerotic and control patients will potentially provide insights into the pathophysiology of atherosclerosis and supply biomarkers for diagnostic purposes. From patients undergoing coronary angiography because of anginal symptoms, we carefully matched 18 patients with severe triple-vessel CAD to 13 control patients without signs of CAD on angiography. All patients were on statin and aspirin treatment. RNA from circulating CD4+ T-cells, CD14+ monocytes, lipopolysaccharide-stimulated monocytes, macrophages and CD34+ progenitor cells was subjected to genome-wide expression analysis. Only CD14+ monocytes demonstrated that a small number of genes involved in activation was overexpressed in control patients, which was verified by real-time polymerase-chain reaction. In this pilot study, cautious matching of patients with severe atherosclerotic CAD with control patients without angiographic signs of coronary atherosclerosis did not reveal differences in transcriptional activity in four out of five different mononuclear cell types. In resting monocytes from patients without overt CAD some inflammatory genes were overexpressed as compared to patients with severe CAD. Large inter-individual variability prevented the use of single differentially expressed genes as biomarkers. Keywords: disease-state analysis In total 153 arrays were analyzed with 6 technical replicates (147 biological samples). CD34+ stem cells, CD4+ T-cells, resting CD14+ monocytes, stimulated monocytes and macrophages were analyzed, all from patient with severe coronary atherosclerosis or controls that had no coronary atherosclerosis as determined angiographically, and which were carefully matched for age and gender.]
Design(s): transcription profiling by array
Publication(s): Schirmer SH, Fledderus JO, van der Laan AM, van der Pouw-Kraan TC, Moerland PD, Volger OL, Baggen JM, Böhm M, Piek JJ, Horrevoets AJ, van Royen N
Suppression of inflammatory signaling in monocytes from patients with coronary artery disease. PubMed:19059264

Sample attribute(s):
Coronary artery disease
2 recorded
no yes
2 recorded
female male
LPS incubation
2 recorded
20 hour 3 hour
1 recorded
peripheral blood
1 recorded
Calcium Antagonists
2 recorded
no calcium channel blocker
family history of CAD
2 recorded
Family History of Coronary Artery Disease no
biopsy site
1 recorded
arterial sheath
patient ID_REF
31 recorded
A8 A27 A6 A12 A7 A20 A11 A36 A35 A41 A10 A32 A25 A31 A2 A39 A15 A30 A9 A34 A38 A4 A1 A3 A13 A33 A19 A37 A5 A22 A24
17 recorded
54 year 56 year 43 year 58 year 66 year 55 year 44 year 62 year 52 year 60 year 49 year 65 year 47 year 42 year 59 year 45 year 37 year
ACE-Inhibitors/ Angiotensin receptor blockers
2 recorded
no yes
admitting diagnosis
2 recorded
Chest pain, unspecified coronary artery disease
1 recorded
acetylsalicylic acid
2 recorded
no Nitrate-based vasodilating agent
3 recorded
no unknown
1 recorded
Homo sapiens
1 recorded
beta blocker
2 recorded
beta-adrenergic antagonist no
culture type
2 recorded
primary culture primary cell
1 recorded
hydroxymethylglutaryl-CoA reductase inhibitor
previous MI
4 recorded
no History of - myocardial infarction unknown No
3 recorded
Non- Ex- Smoker
1 recorded
cell type
3 recorded
CD34 CD4 CD14
cell type2
4 recorded
macrophage common lymphoid progenitor T-lymphocyte monocyte
Contact(s): Stephan SchirmerN RoyenStephan SchirmerAnton HorrevoetsJoost Fledderus
Release Date: 12/1/2008
Submission Date:
Metadata Downloads Download Study Metadata as ISAtabDownload Study Metadata as ISAtab
Assay Downloads
transcription profiling using DNA microarray
153 assays